Filter
Top Products
4. Operation
4.3
Conversion
T%
AU OD
OD
table T% to AU
(3 mm cell)
(10 mm cell)
and OD
1
5
10
15
20
25
30
32
35
40
45
50
55
60
63
65
70
75
79
80
85
89
90
95
95.5
97.7
2.000
1.301
1.000
0.824
0.699
0.602
0.523
0.500
0.456
0.398
0.347
0.301
0.260
0.222
0.200
0.186
0.155
0.125
0.100
0.097
0.070
0.050
0.046
0.022
0.020
0.010
6.667
4.337
3.333
2.747
2.330
2.007
1.743
1.667
1.520
1.327
1.157
1.003
0.867
0.740
0.667
0.620
0.517
0.417
0.333
0.323
0.233
0.167
0.153
0.073
0.067
0.033
2.000
1.301
1.000
0.824
0.699
0.602
0.523
0.500
0.456
0.398
0.347
0.301
0.260
0.222
0.200
0.186
0.155
0.125
0.100
0.097
0.070
0.050
0.046
0.022
0.020
0.010
4.4 Start-up
Note:
Always ensure that all liquid passing through the flow cell
1.
2.
3.
are degassed to prevent any air bubble formation in the cell.
Liquids must be filtered to remove any particulate material
and prevent any blockage.
Check that the monitor is correctly installed and that the sample
cell is filled with the appropriate eluent. The reference cell may
be left closed with air in the cell unless the UV-1 is used with
eluents showing appreciable UV-absorption. In this case the
reference cell should be filled with eluent.
If the instrument has been switched off, turn on the mains switch
(Fig. 5:16) and refer to Section 4.5, to restabilize the instrument.
Check that the appropriate filter and converter or aperture are in
place and fully inserted.
4.5
Stabilization
At normal laboratory temperatures the UV-1 requires 2 hours to
time
stabilize sufficiently. When the UV- 1 is in constant use, it is
recommended that it remains switched on. UV- 1 can be switched off
when not in use for periods of one week or more.
For coldroom operation below 10 °C, install UV-1 in the coldroom at
the desired running temperature at least 12 hours before the start of a
run. This is necessary to allow the instrument housing to equilibrate
to the temperature of the coldroom. Once equilibration has taken
place, the stabilisation times given for normal temperatures are valid.
16