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38 PIXIS System Manual Version 2.C
Experiment Setup Main tab (Acquisition|Experiment Setup…):
Exposure Time: 100 ms
Accumulations & Number of Images: 1
Experiment Setup ROI tab (Acquisition|Experiment Setup…): Use this
function to define the region of interest (ROI).
Imaging Mode: Select this mode if you are running WinSpec.
Clicking on Full loads the full size of the chip into the edit boxes.
Experiment Setup Timing tab (Acquisition|Experiment Setup…):
Timing Mode: Free Run
Shutter Control: Normal
Safe Mode vs. Fast Mode: Fast
Confirming the Setup
1. If you are using WinView/32 and the computer monitor for focusing, select
Focus from the Acquisition menu. Successive images will be sent to the
monitor as quickly as they are acquired.
2. Adjust the lens aperture, intensity scaling, and focus for the best image as
viewed on the computer monitor. Some imaging tips follow:
Begin with the lens blocked off and then set the lens at the smallest
possible aperture (largest f-stop number).
Make sure there is a suitable target in front of the lens. An object with
text or graphics works best.
Adjust the intensity scaling (by clicking the 5%-95% button at the
bottom left corner of the data window) and adjust the lens aperture until
a suitable setting is found. Once you’ve determined that the image is
present, select a lower setting for better contrast. Check the brightest
regions of the image to determine if the A/D converter is at full-scale. A
16-bit A/D is at full scale when the brightest parts of the image reach an
intensity of 65535. Adjust the aperture to where it is just slightly smaller
(higher f-stop) than the setting where maximum brightness on any part of
the image occurs.
Set the focus adjustment of the lens for maximum sharpness in the
viewed image.
In the case of a camera with an F-mount, the camera lens adapter itself
also has a focus adjustment. If necessary, this focus can be changed to
bring the image into range of the lens focus adjustment. See “F-Mount
Adapter Focusing Procedure”, page 110.
3. After you have focused the camera, you can stop Focus mode, continue
Focus mode, begin Acquire mode, or wait for the CCD to reach the
operating temperature before going to Acquire mode.