42 PIXIS System Manual Version 2.C
the problem is fixed, stop acquisition or continue to the “Focusing” topic on
page 42; otherwise, stop data acquisition and continue to Step g.
If you do not hear a shutter operating and you have done Steps a-e, stop data
acquisition and continue to Step g.
g. Make sure the spectrograph has an entrance slit shutter. An externally mounted
shutter is easily confirmed. Verifying an internally mounted shutter requires
access to the inside of the spectrograph: refer to the spectrograph manual for
instructions.
h. Check to see if the PIXIS has a shutter. Loosen and remove the camera from the
spectrograph. If you see the CCD when you look into the front of the camera, the
camera does not have an internal shutter. Re-mount the camera to the spectrograph.
i. If the camera and spectrograph both have shutters, permanent damage to the
shutter driver may occur. You can either disconnect and remove the entrance slit
shutter or control it via a Princeton Instruments SHC-EXT Control Box. To
disconnect and remove an entrance slit shutter:
External Slit Shutter: Disconnect the shutter cable and remove the shutter
assembly from the spectrograph entrance port.
Internal Slit Shutter: Disconnect the shutter cable. Removing an internally
mounted shutter requires access to the inside of the spectrograph: refer to the
spectrograph manual for removal instructions.
5. If you need more help, contact Customer Support. See page 132 for contact
information.
Focusing
The mounting hardware provides two degrees of freedom, focus and rotation. In this
context, focus means to physically move the camera back and forth through the focal
plane of the spectrograph. The approach taken is to slowly move the camera in and out of
focus and adjust for optimum while watching a live display on the monitor, followed by
rotating the camera and again adjusting for optimum. The following procedure, which
describes the focusing operation with an Acton SP-2300i spectrograph, can be easily
adapted to other spectrographs. For IsoPlane SCT-320 related focusing information, see
“IsoPlane SCT-320 Spectrograph” on page 44.
If the PIXIS has an internal shutter, DO NOT USE the Shutter connector to drive a
spectrograph entrance slit shutter. If both the camera and the spectrograph have
shutters, the entrance slit shutter will have to be removed or a Princeton Instruments
SHC-EXT Shutter Control Box may be used to control the opening and closing of the
entrance slit shutter. See the spectrograph manual.
Acton Series Spectrograph
1. Mount a light source such as a mercury pen-ray type in front of the entrance slit of
the spectrograph. Any light source with line output can be used. Standard fluorescent
overhead lamps have good calibration lines as well. If there are no "line" sources
available, it is possible to use a broadband source such as tungsten for the alignment.
If this is the case, use a wavelength setting of 0.0 nm for alignment purposes.
Caution