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Chapter 5 Operation 41
41
Experiment Setup Main tab (Acquisition|Experiment Setup…):
Exposure Time: 100 ms
Accumulations & Number of Images: 1
Experiment Setup ROI tab (Acquisition|Experiment Setup…): Use this
function to define the region of interest (ROI).
Spectroscopy Mode: Selected
Clicking on Full loads the full size of the chip into the edit boxes.
Experiment Setup Timing tab (Acquisition|Experiment Setup…):
Timing Mode: Free Run
Shutter Control: Normal
Safe Mode vs. Fast Mode: Fast
Setting the Spectrograph Parameters
1. Define Spectrograph dialog (Spectrograph|Define): Click on
Install/Remove Spectrograph, highlight the appropriate spectrograph name in the
Supported Spectrographs list (for example, Acton SP-300i for an Acton
SP-2300i or Acton SCT320 for an IsoPlane), and click on Install Selected
Spectrograph.
2. Move Spectrograph dialog: Choose the grating to be move, and then set it to
500 nm if using a mercury lamp or to 0.0 nm if using a broadband source.
Confirming the Setup
1. Turn on the light source at the spectrograph entrance slit.
2. In WinSpec, select Focus (on the Acquisition menu or on the Experiment Setup
dialog) to begin data accumulation. Depending on the display settings, you should
see either a spectral band (image) or a graph. Background noise will decrease as the
camera cools to its default temperature.
3. Turn off the light source. The data displayed should change to a background noise
pattern or low intensity graph. If this occurs, you have confirmed that light entering
the spectrograph is being seen by the camera. Skip Steps 4-5 and continue to the
“Focusing” topic on page 42.
4. If there is no difference between the data displayed when the light source is on or off:
a. Verify that the light source has power and is turned on.
b. Verify that the entrance slit is open at least 10 µm.
c. Check the Exposure Time (Experiment Setup Timing tab).
d. Confirm that Shutter Control is set to Normal (Experiment Setup Timing tab).
e. Check the shutter cable connections.
f. Verify shutter operation. You should hear the shutter open and close while
running in Focus mode.
If you hear a shutter operating and you have done Steps a-e, turn the light source
on, wait a minute and then turn the light off while you view the data display. If