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VI Basic Operations MICROSCOPE
6 Using the Microscope Control
- 139 -
Focus (Adjusts focus)
Compatible only with the Nikon 90i microscope.
1
）
Set the coarse/fine select icon to coarse motion mode. (The arrow icon changes
shape for coarse motion and fine motion modes.
for coarse motion mode, for
fine motion mode.)
2
）
Use the Up icon
and the Down icon to adjust the focus position.
3
）
Finally, to fine adjust, set the coarse/fine select icon
to fine motion mode and use the
Up icon
and the Down icon to adjust the focus position.
NOTE:
* Information on the height of the current Z position is displayed on the
"microscope status display/microscopy setup menu" (MIC Info menu).
* To replace specimens, for example, select the escape icon
. The stage
will descend to a height at which you can replace specimens. Select the
escape icon
again after replacing specimens to return the stage to its
original position.
* If the exposure time is long and you have difficulty fine-adjusting the focus
position, use the "CAM" menu to adjust the exposure time. For more
information, refer to the explanation column of the "CAM" menu.
<Using the autofocus function>
When this function is selected, focus is adjusted automatically. Before using this function, please
read the separate manual entitled "90i Reference: Autofocus." Also, refer to Section 6.3,
"Autofocus Precautions," in this manual.
1
）
Select the autofocus (AF) icon .
2） The message "Now Focusing" will be displayed at the upper right part of the screen,
indicating that the autofocus function is being executed. During this time, the image
display remains frozen.
3） When autofocus is complete, the message "Complete" is displayed, and the frozen image
display is updated to show the current image.
NOTE:
* While autofocus is underway, you cannot use any other menu.
* After autofocus is complete, a completed status code may be displayed. For
detailed information about the code, refer to the instruction manual for your
microscope.
* If this is the first time you have used autofocus after replacing samples,
make sure the magnification of the objective is x4 or less. (At higher
magnifications, the autofocus range is narrowed.) Make sure the target to
be inspected is at center of the view field.
* Use the autofocus function under scene mode "BF," display mode "1.3MP,"
and exposure mode "PROG: 1/120–1/350." Make sure the focus indicator is
about 350 or more when the sample is approximately in focus.