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VI Basic Operations MICROSCOPE
6 Using the Microscope Control
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6.3
Autofocus Precautions
Before using autofocus, check your microscope for the following. Disregarding
these precautions may result in damage to the objective or sample.
Precautions for samples
1） The autofocus function may be used with samples mounted on specimen slides with cover
glass in the sizes indicated below.
• Thickness:
0.9 mm to 1.7 mm, inclusive [thickness of slide glass + thickness of cut piece (not
including the cover glass)]
• Slide glass:
Complies with JIS R3703 or ISO 8037.
• Cover glass:
Complies with JIS R3702 or ISO 8255.
[JIS R3702]
Thickness: 0.12–0.17 mm for No. 1; 0.15–0.18 mm for No.1-S
[ISO 8255]
Thickness: 0.13–0.17 mm for No. 1; 0.15–0.17 mm for No.1-H
2） The autofocus function may force contact between the sample and the tip of the objective,
resulting in damage to both. For samples like the ones shown below, adjust focus with the
focusing handle rather than relying on autofocus.
• A sample with sealing agent rising excessively above the cover-glass top surface.
• A sample whose sealing agent is damp, not completely dry
• A sample with sealing agent adhering to the surface of the cover glass
• A sample whose cover glass is sticking out from the slide glass
Slide glass
Cut piece
Cover glass
1.7 mm or less